The Reversible Inactivation of Rat-Liver Arginase at Low pH
نویسندگان
چکیده
منابع مشابه
Molecular characteristics of rat liver arginase.
The molecular weight of approximately 1,500-fold purified arginase from rat liver (specific activity = 19,500 pmoles of urea per min at 25’ per mg of protein nitrogen) was determined by the method of sedimentation equilibrium to be 118,000. The sedimentation velocity constant is 6.1 S, the diffusion coefficient 5.2 X 10U7 cm2 per sec. Alteration in pH, removal of Mn2+, and replacement of Mn2+ b...
متن کاملOn molecular sizes of rat liver arginase.
Arginase exists as 2 or more molecular species in the size profile of the soluble macromolecules of rat liver in the absence of added Mn@. The principal species is that of approximately 120,000 molecular weight, as previously re ported by others. In addition, after activation by Mn@ , there are detectable small but significant amounts of arginase of about 30,000—40,000 molecular weight, presu...
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Introduction: Several supra spinal areas such as rostral ventrolateral medulla (RVLM) area are involved in basic cardiovascular regulation. The Kolliker— Fuse nucleus (KF) is located in pons and is heavily connected with RVLM. The cardiovascular effect of KF nucleus has been shown and it is suggested that KF is involved in sympathetic vasomotor tone and basic cardiovascular regulation. Therefor...
متن کاملZn2+-dependent reversible inactivation of rat liver phosphofructokinase-1. Purification of the inactivating protein and characterization of the inactivation reaction.
A protein has been purified from rat liver (about 5 mg from 100 g) which inactivates rat liver phosphofructokinase-1. According to dodecyl sulfate gel electrophoresis the protein consists of a single peptide chain with a Mr of 19,000. The inactivation of phosphofructokinase-1 by this protein results from a dissociation of phosphofructokinase-1 into its inactive protomers (Mr = 82,000). The inac...
متن کاملIn vitro analysis of the rat liver-type arginase promoter.
Genes for urea cycle enzymes including liver-type arginase are expressed mainly in the liver and are regulated developmentally, nutritionally, and hormonally in a coordinated manner. The promoter region of the rat arginase gene was investigated with an in vitro transcription system using nuclear extracts prepared from rat tissues. Accurate initiation of the transcription in liver nuclear extrac...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1972
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1972.tb01809.x